Correlation Between flow Cytometry and Transmission Electron Microscopy
نویسندگان
چکیده
Flow cytometry techniques together with morphologic studies were used to perform multiparametric analyses with the aim of studying the dynamic aspects of cell cultures derived from CE44 teratocarcinoma embryoid bodies. The intrinsic cell parameters studied by flow cytometry were size (FALS) and cytoplasmic complexity (ISS). Our results show that CE44 teratocarcinoma yields monolayers whose cells show a marked morphological heterogeneity that can be grouped according to flow cytometric criteria into four populations. These cytometric findings can be correlated with differences in cytoplasmatic traits observed in the electron microscopic study.
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